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What type of media is recommended for the recovery of Mycobacterium haemophilum?


A) Sheep blood agar (SBA)
B) Chocolate
C) Polysaccharide egg antigen (PEA)
D) Lowenstein-Jensen (LJ)

E) B) and C)
F) A) and B)

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What is the purpose of the digestion-decontamination processing of specimens submitted for mycobacterial culture?


A) To release all bacteria in the sample
B) To make the specimen easier to plate
C) To allow chemical decontaminants to kill nonmycobacterial organisms
D) To liquefy the outer cell wall of the mycobacteria so that they will grow faster

E) A) and D)
F) A) and C)

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What Mycobacterium species fails to grow in vitro?


A) Mycobacterium tuberculosis
B) M. leprae
C) M. avium
D) M. marinum

E) A) and B)
F) A) and D)

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What media are recommended for routine culturing of specimens for the recovery of acid-fast bacilli?


A) Lowenstein-Jensen and mycobacterial growth indicator tube
B) Middlebrook 7H10 and cooked meat broth
C) Sabouraud dextrose and LIM broth
D) Thiosulfate-citrate-bile salts-sucrose (TCBS) and thioglycollate broth

E) A) and B)
F) A) and C)

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A child visits his doctor because he has had a fever and a nonproductive cough.The child is also short of breath.The doctor orders a routine sputum culture and an acid-fast bacillus (AFB) culture and smear.The smears shows red organisms arranged in ropes.What is the most probable cause of this child's fever and cough?


A) Mycobacterium leprae
B) M. avium
C) M. intracellulare
D) M. tuberculosis

E) A) and C)
F) None of the above

Correct Answer

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Decontaminating agents used in the decontamination-digestion process include all of the following EXCEPT:


A) Sodium hydroxide
B) N-acetyl-1-cysteine
C) Benzalkonium chloride
D) Hydrochloric acid

E) A) and B)
F) All of the above

Correct Answer

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The Centers for Disease Control and Prevention (CDC) recommends all the following drugs for the treatment of Mycobacterium tuberculosis EXCEPT:


A) Ethambutol
B) Isoniazid
C) Rifampicin
D) Ciprofloxacin

E) None of the above
F) A) and B)

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What is the optimum growth time for most mycobacteria associated with disease?


A) 2 to 6 weeks
B) 1 to 2 weeks
C) 5 to 7 days
D) 3 to 5 days

E) A) and D)
F) B) and C)

Correct Answer

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How long does it take to detect Mycobacterium spp.with the BACTEC method?


A) Less than 2 weeks
B) 3 weeks
C) 4 weeks
D) 6 weeks

E) C) and D)
F) All of the above

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Middlebrook 7H10 and 7H11 media are enriched with all of the following EXCEPT:


A) Oleic acid
B) Bovine albumin
C) Heme
D) Beef catalase

E) B) and D)
F) B) and C)

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What component of mycobacteria is used for identification in the nucleic acid hybridization assays?


A) DNA
B) Ribosomal RNA
C) Transfer RNA
D) Messenger RNA

E) All of the above
F) A) and C)

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If a person has had tuberculosis during his or her lifetime,how likely is it that the person will get the disease again?


A) 5% to 15%
B) 20% to 25%
C) 1%
D) 50%

E) All of the above
F) A) and B)

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A patient with Hodgkin's disease is feeling bad,so he pays a visit to his physician.The physician's clinical examination reveals submandibular lymphadenitis,subcutaneous nodules,painful swellings,ulcers progressing to abscesses,and draining fistulas.What organism is probably producing these symptoms?


A) Mycobacterium tuberculosis
B) M. avium
C) M. gordonae
D) M. haemophilum

E) A) and B)
F) A) and C)

Correct Answer

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